With the world’s population expected to exceed nine billion by 2050, scientists are working to develop new ways to meet rising global demand for food, energy and water without increasing the strain on natural resources and environmental pressure. Organizations, including the World Bank and the U.N. Food and Agriculture Organization, as well as the EU F2F and Green Deal strategies, are calling for more innovation to address the challenges of the agri-food sector. The development of nano-based techniques and nanostructured materials for agriculture has been started very recently; they will be implemented within the evolving science of precision agriculture, in which farmers use technology to target their use of water, fertilizer, plant protection products and other inputs. A second, broad potential application concerns the issues of reduction and valorization of agri-food waste, where nano- and biotechnologies could offer valuable approaches and solutions. The introduction of innovative technologies in agricultural practices still needs to deepen basic and applied knowledge. However, several promising results have been achieved so far. A huge development is taking place in this sector. Therefore, nanotech applications currently under development will soon be overtaken by other ideas that are expected to contribute to solving several issues in agriculture and sustainable use of resources. NanoInnovation 2025 hosts the workshop “Nano-enabled Agriculture” co-organized by the Universities of Tuscia, Udine and the Turin Politecnico, along with scientific support of many international research institutes. The workshop will be a forum for discussing the perspective of nanotechnologies and other enabling technologies for the valorization of the primary sector among the stakeholders and scientific researchers.